Datasheet

Caveolin-1 Antibody
CATALOG NUMBER: 8219

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Horse: (100%), Sheep: (100%), Bovine: (100%), Pig: (100%), Chicken: (88%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Caveolin-1 antibody can be used for detection of Caveolin-1 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 20 kDa

Observed: 21 kDa
SPECIFICITY:At least two isoforms of Caveolin-1 are known to exist; this antibody will detect only the largest isoform.
IMMUNOGEN:Caveolin-1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human Caveolin-1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caveolin-1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caveolin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caveolin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caveolin-1 Antibody: Caveolin-1, BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21
ACCESSION NO.:NP_001744
PROTEIN GI NO.:15451856
OFFICIAL SYMBOL:CAV1
GENE ID:857

Background

BACKGROUND:Caveolin-1 Antibody: Caveolin-1 is a scaffolding protein that is the main component of the caveolae plasma membranes found in most cell types (1). The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression (2). The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade (3). Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy (4).
REFERENCES: 1) Glenney JR Jr. The sequence of human caveolin reveals identity with VIP21, a component of transport vesicles. FEBS Lett. 1992; 314:45-8.
2) Wary KK, Mariotti A, Zurzolo C, et al. A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growth. Cell 1998; 94:625-34.
3) Capozza F, Williams TM, Schubert W, et al. Absence of caveolin-1 sensitizes mouse skin to carcinogen-induced epidermal hyperplasia and tumor formation. Am. J. Pathol. 2003; 162:2029-39.
4) Kim CA, Delepine M, Boutet E, et al. Association of a homozygous nonsense caveolin-1 mutation with Berardinelli-Seip congenital lipodystrophy. J. Clin. Endocrinol. Metab. 2008; 93:1129-34.

For Research Use Only