Caspase-9 Antibody Cat. No.: 2073

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat (50%).
IMMUNOGEN: Anti-Caspase 9 antibody (2073) was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9.

The immunogen is located within amino acids 290-340 of Caspase 9.
TESTED APPLICATIONS: ELISA, ICC, IF, IP, WB
APPLICATIONS: WB: 1 μg/mL; ICC: 2- 5 μg/mL; IF: 5-20 μg/mL.

Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry, Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 46kD

Observed: 46 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar CASPASE 9 expression profile in human cell lines detected by two independent anti-CASPASE 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  CASPASE 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD). 2073 can detect human and mouse isoform.

psi-iconProperties
PURIFICATION:Caspase-9 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CASP9
ALTERNATE NAMES:Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
ACCESSION NO.:P55211
PROTEIN GI NO.:28558771
GENE ID:842
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
REFERENCES:1) Duan et al. J Biol Chem 1996;271:16720-4.
2) Srinivasula et al. J Biol Chem 1996;271:27099-106.

ANTIBODIES FOR RESEARCH USE ONLY.

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