Caspase-9 Antibody Cat. No.: 2071

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9.

The immunogen is located within amino acids 30-80 of Caspase 9.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: WB: 0.5-2 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
SPECIFICITY: It has no cross response to other members in caspase family.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1204 - K562 Cell Lysate
 3) Cat. No. 1466 - Rat Spleen Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 46kD

Observed: 46kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar Caspase 9 expression profile in human cell lines detected by two independent anti-Caspase 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  Caspase 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human Caspase 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse Caspase 9 has one isoform (454aa, 50kD) and rat Caspase 9 also has one isoform (454aa, 50kD). 2071 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:Caspase-9 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Caspase-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CASP9
ALTERNATE NAMES:Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
ACCESSION NO.:P55211
PROTEIN GI NO.:28558771
GENE ID:842
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
REFERENCES:1) Duan et al. J Biol Chem 1996;271:16720-4.
2) Srinivasula et al. J Biol Chem 1996;271:27099-106.
3) Li et al. Cell 1997;91:479-89.
4) Pan et al. J Biol Chem 1998;273:5841-5 (RD1299).

ANTIBODIES FOR RESEARCH USE ONLY.

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