Datasheet

Caspase-7 Antibody
CATALOG NUMBER: 3465

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:Casp-7 antibody can be used for the detection of Caspase-7 by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
SPECIFICITY:Depending on cell lines or tissues used, other cleavage products may be observed.
IMMUNOGEN:Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-7.

The immunogen is located within the last 50 amino acids of Caspase-7.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-7 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-7 Antibody: MCH3, CMH-1, LICE2, CASP-7, ICE-LAP3, MCH3, Caspase-7, Apoptotic protease Mch-3
ACCESSION NO.:P55210
PROTEIN GI NO.:1730092
OFFICIAL SYMBOL:CASP7
GENE ID:840

Background

BACKGROUND:Caspase-7 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3. Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms. Caspase-7 activity can be directly inhibited by XIAP expression.
REFERENCES: 1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2) Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3) Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4) Juan TSC, McNiece IK, Argento JM, et al. Identification and mapping of Casp7, a cysteine protease resembling CPP32b, Interleukin-1b converting enzyme, and CED-3. Genomics 1997; 40:86-93.

For Research Use Only