Datasheet

Caspase-5 Antibody
CATALOG NUMBER: 3455

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Casp-5 antibody can be used for the detection of Caspase-5 by Western blot at 0.5 to 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1225 - Ramos Cell Lysate
 2) Cat. No. 1314 - Human Thymus Tissue Lysate
 3) Cat. No. 1306 - Human Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 48 kDa

Observed: 48 kDa
SPECIFICITY:Depending on cell lines or tissues used, other cleavage products may be observed.
IMMUNOGEN:Caspase-5 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-5.

The immunogen is located within amino acids 40 - 90 of Caspase-5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-5 Antibody: ICH-3, ICEREL-III, ICE(rel)III, ICH3, Caspase-5, Protease ICH-3, CASP-5
ACCESSION NO.:P51878
PROTEIN GI NO.:212276423
OFFICIAL SYMBOL:CASP5
GENE ID:838

Background

BACKGROUND:Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.
REFERENCES: 1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
3) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
4) Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1beta. Mol. Cell. 2002; 10:417-26.

For Research Use Only