Datasheet

Caspase-3 Antibody
CATALOG NUMBER: 3445

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Rabbit: (100%), Rat: (100%), Mouse: (87%), Bovine: (92%)
TESTED APPLICATIONS:ELISA, IHC-P
APPLICATIONS:Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
PREDICTED MOLECULAR WEIGHT:30 kDa
SPECIFICITY:At least two isoforms of Caspase-3 are known to exist.
IMMUNOGEN:Caspase-3 antibody was raised against a 17 amino acid synthetic peptide near the center of human Caspase-3.

The immunogen is located within amino acids 70 - 120 of Caspase-3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3
ACCESSION NO.:NP_004337
PROTEIN GI NO.:14790119
OFFICIAL SYMBOL:CASP3
GENE ID:836

Background

BACKGROUND:Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.
REFERENCES: 1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74
2) Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3) Wolf BB and Green DR. Suicidal tendencies: apoptotic cell death by caspase family proteinases. J. Biol. Chem. 1999; 274:20049-52.
4) Slee EA, Adrain C, and Martin SJ. Serial killers: ordering caspase activation events in apoptosis. Cell Death Diff. 1999; 6:1067-74.

For Research Use Only