Datasheet

Caspase-14 Antibody
CATALOG NUMBER: 2509

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:Caspase-14 antibody can be used for the detection of caspase-14 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1304 - Human Liver Tissue Lysate
 3) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 28 kDa

Observed: 27 kDa
IMMUNOGEN:Caspase-14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human caspase-14.

The immunogen is located within the last 50 amino acids of Caspase-14.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-14 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-14 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-14 Antibody: MICE, mini-ICE, Caspase-14, Mini-ICE, CASP-14
ACCESSION NO.:ACA63845
PROTEIN GI NO.:169666460
OFFICIAL SYMBOL:Casp14
GENE ID:23581

Background

BACKGROUND:Caspase-14 Antibody: Caspases are a family of cysteine proteases that can be divided into apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18. Caspase-14 is highly expressed in embryonic but not adult tissues. It is processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in the keratinocyte terminal differentiation, which is important for the formation of the skin barrier.
REFERENCES: 1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2) Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
4) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

For Research Use Only