Datasheet

Caspase-13 Antibody
CATALOG NUMBER: 2245

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:IHC-P
APPLICATIONS:Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 μg/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1209 - HL60 Cell Lysate
 2) Cat. No. 1403 - Mouse Brain Tissue Lysate
 3) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:43 kDa
IMMUNOGEN:Caspase-13 antibody was raised against a 15 amino acid peptide near the amino terminus of human Caspase-13.

The immunogen is located within amino acids 80 - 130 of Caspase-13.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-13 Antibody is Ion exchange chromatography purified.
PHYSICAL STATE:Liquid
BUFFER:Caspase-13 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Caspase-13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-13 Antibody: CASP13, Caspase-13, Evolutionary related interleukin-1-beta-converting enzyme, CASP-13
ACCESSION NO.:O75601
PROTEIN GI NO.:10720034
OFFICIAL SYMBOL:CASP4
GENE ID:338039

Background

BACKGROUND:Caspase-13 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 beta Converting Enzyme) and caspase-13. Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.
REFERENCES: 1) Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem 1998;273:15702-7 (WD0500)

For Research Use Only