Datasheet

Caspase-12 Antibody (Small)
CATALOG NUMBER: 3197

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (93%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in mouse and rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 1464 - Rat Liver Tissue Lysate
 3) Cat. No. 10-201 - Human Liver Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 46 kDa (uncleaved)

Observed: 38 kDa
IMMUNOGEN:Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12.

The immunogen is located within the last 50 amino acids of Caspase-12 .
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-12 Antibody (Small) is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-12 antibody (Small) can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-12 Antibody (Small): Caspase-12, CASP-12
ACCESSION NO.:CAA73532
PROTEIN GI NO.:2094806
OFFICIAL SYMBOL:Casp12
GENE ID:12364

Background

BACKGROUND:Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
REFERENCES: 1) Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
2) Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
3) Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.

For Research Use Only