Datasheet

Caspase-12 Antibody (Large)
CATALOG NUMBER: 3195

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 1401 - Mouse Heart Tissue Lysate
 3) Cat. No. 10-501 - Human Heart Tissue Slide
 4) Cat. No. 1461 - Rat Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 46 kDa

Observed: 46 kDa
SPECIFICITY:Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.
IMMUNOGEN:Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.

The immunogen is located within amino acids 170 - 220 of Caspase-12.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-12 (Large) antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-12 (Large) Antibody: Caspase-12, CASP-12
ACCESSION NO.:CAA73532
PROTEIN GI NO.:2094806
OFFICIAL SYMBOL:Casp12
GENE ID:12364

Background

BACKGROUND:Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
REFERENCES: 1) Nakagawa T, Zhu H, Morishima N, et al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
2) Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
3) Van de Craen M, Vandenabeele P, Declercq W, et al. Characterization of seven murine caspase family members. FEBS Lett. 1997; 403:61-9

For Research Use Only