Datasheet

Caspase-12 Antibody
CATALOG NUMBER: 2327

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 1306 - Human Spleen Tissue Lysate
 3) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 4) Cat. No. 1404 - Mouse Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 46 kDa

Observed: 55 kDa
IMMUNOGEN:Caspase-12 antibody was raised against a 17 amino acid peptide near the center of murine Caspase-12.

The immunogen is located within amino acids 90 - 140 of Caspase-12.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-12 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-12 Antibody: Caspase-12, CASP-12
ACCESSION NO.:CAA73532
PROTEIN GI NO.:2094806
OFFICIAL SYMBOL:Casp12
GENE ID:12364

Background

BACKGROUND:Caspase-12 Antibody: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta.
REFERENCES: 1) Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
2) Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
3) Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.

For Research Use Only