Caspase-10 Antibody Cat. No.: 1128

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Caspase-10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Caspase-10.

The immunogen is located within amino acids 240 - 290 of Caspase-10.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: Caspase-10 antibody can be used for detection of caspase-10 by Western blot 1 μg/mL. A 59 kDa band can be detected.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Antibody only recognizes FLICE2 form of caspase-10.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 1202 - A431 Cell Lysate
 4) Cat. No. 1204 - K562 Cell Lysate

psi-iconProperties
PURIFICATION:Caspase-10 Antibody is Antibody is DEAE purified.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Caspase-10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Caspase-10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CASP10
ALTERNATE NAMES:Caspase-10 Antibody: MCH4, Caspase-10, Apoptotic protease Mch-4, CASP-10
ACCESSION NO.:AAD28402
PROTEIN GI NO.:4731237
GENE ID:843
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Caspase-10 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.
REFERENCES:1) Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1β-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 1997;272:6578-6583
2) Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM, Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469
3) Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997;326:1-16 (RD1299)

ANTIBODIES FOR RESEARCH USE ONLY.

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