Datasheet

Caspase-1 Antibody
CATALOG NUMBER: 3459

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (88%), Pig: (81%), Horse: (81%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
SPECIFICITY:Depending on cell lines or tissues used, other cleavage products may be observed.
IMMUNOGEN:Caspase-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-1.

The immunogen is located within the last 50 amino acids of Caspase-1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Caspase-1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Caspase-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1
ACCESSION NO.:NP_150634
PROTEIN GI NO.:15431328
OFFICIAL SYMBOL:CASP1
GENE ID:834

Background

BACKGROUND:Caspase-1 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Caspase-1 was initially identified as an IL-1β-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
REFERENCES: 1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2) Zhivotovsky B and Orrenius S. Caspase-2 function in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3.
4) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.

For Research Use Only