Datasheet

CASKIN1 Antibody
CATALOG NUMBER: 6991

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (71%), Rat: (71%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CASKIN1 antibody can be used for detection of CASKIN1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 157 kDa

Observed: 160 kDa
SPECIFICITY:CASKIN1 antibody is predicted to not cross-react with other CASKIN family members.
IMMUNOGEN:CASKIN1 antibody was raised against a 17 amino acid synthetic peptide near the center of human CASKIN1.

The immunogen is located within amino acids 720 - 770 of CASKIN1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CASKIN1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CASKIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CASKIN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CASKIN1 Antibody: ANKS5A, KIAA1306, Caskin-1, CASK-interacting protein 1
ACCESSION NO.:NP_065815
PROTEIN GI NO.:18079216
OFFICIAL SYMBOL:CASKIN1
GENE ID:57524

Background

BACKGROUND:CASKIN1 Antibody: Calcium/calmodulin-dependent serine protein kinase (CASK) is a conserved multi-domain scaffolding protein involved in brain development, synapse formation, and establishment of cell polarity. CASKINs (CASK interacting proteins) interact with CASK and is thought to play a role in CASK function, specifically by coupling CASK to distinct downstream effectors. CASKIN1 is a multidomain protein containing six N-terminal ankyrin repeats, one SH3 domain, and two sterile alpha motif domains followed by a long proline-rich sequence and a short conserved C-terminal domain. CASKIN1 coassembles with CASK on the cytoplasmic tail of neurexin 1, suggesting that CASKIN and CASK coat the cytoplasmic tails of neurexins and other cell-surface proteins.
REFERENCES: 1) Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
2) Weng YL, Liu N, DiAntonio A, et al. The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance. Neurosci. 2011; 31:4421-33.
3) Tabuchi K, Biederer T, Butz S, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with CASKIN1, a novel CASK-binding protein. J. Neurosci. 2002; 22:4264-73.

For Research Use Only