Datasheet

CARMA3 Antibody
CATALOG NUMBER: 3193

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (81%)
TESTED APPLICATIONS:ELISA, IHC-P
APPLICATIONS:CARMA3 antibody can be used for detection of CARMA3 by immunohistochemistry at 5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 10-401 - Human Kidney Tissue Slide
SPECIFICITY:CARMA3 antibody is human specific. At least three isoforms of CARMA3 are known to exist; this antibody will only detect isoform 1. CARMA3 antibody is predicted not to cross-react with other CARMA proteins.
IMMUNOGEN:CARMA3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CARMA3.

The immunogen is located within the first 50 amino acids of CARMA3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CARMA3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CARMA3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CARMA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CARMA3 Antibody: BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3
ACCESSION NO.:NP_055365
PROTEIN GI NO.:51093861
OFFICIAL SYMBOL:CARD10
GENE ID:29775

Background

BACKGROUND:CARMA3 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-κB activation. When expressed in cells, this protein binds to BCL10 and activates NF-κB Recent experiments have shown that CARMA3 is required for EGF-induced NF-κB activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.
REFERENCES: 1) Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
2) Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappa B activation. FEBS Lett. 2001; 496:121-7.
3) Wang L, Guo Y, Huang WJ, et al. Card10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappaB. J. Biol. Chem. 2001; 276:21405-9.
4) Jiang T, Grabiner B, Zhu Y, et al. CARMA3 is crucial for EGFR-induced activation of NF-kappaB and tumor progression. Cancer Res. 2011; 71:2183-92.

For Research Use Only