Datasheet

CARM1 Antibody
CATALOG NUMBER: 7219

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CARM1 antibody can be used for detection of CARM1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 67 kDa
IMMUNOGEN:Rabbit polyclonal CARM1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CARM1.

The immunogen is located within amino acids 470 - 520 of CARM1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CARM1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CARM1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CARM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CARM1 Antibody: PRMT4, PRMT4, Histone-arginine methyltransferase CARM1, Coactivator-associated arginine methyltransferase 1
ACCESSION NO.:NP_954592
PROTEIN GI NO.:40288288
OFFICIAL SYMBOL:CARM1
GENE ID:10498

Background

BACKGROUND:CARM1 Antibody: Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation. CARM1 functions as a transcriptional co-activator for various nuclear receptors and NF-κB. It has also been shown to methylate histone H3 arginines, inhibiting the binding of corepressors and protecting chromatin from deacetylation, thereby facilitating transcription.
REFERENCES: 1) Frankel A, Yadav N, Lee J, et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J. Biol. Chem. 2002; 277:3537-43.
2) Wolf SS. The protein arginine methyltransferase family: an update about function, new perspectives and the physiological role in humans. Cell Mol. Life Sci. 2009; 66:2109-21.
3) Lee DY, Teyssier C, Strahl BD, et al. Role of protein methylation in regulation of transcription. Endocr. Rev. 2005; 26:147-70.
4) Covic M, Hassa PO, Saccani S, et al. Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB dependent gene expression. EMBO J. 2005; 24:85-96.

For Research Use Only