Datasheet

CARD9 Antibody
CATALOG NUMBER: 2515

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (88%), Rat: (82%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:CARD9 antibody can be used for detection of CARD9 by Western blot at 2.5 μg/mL. A band at approximately 59 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1216 - PC-3 Cell Lysate
 2) Cat. No. 1217 - MDA-MB-361 Cell Lysate
 3) Cat. No. 1204 - K562 Cell Lysate
 4) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:59 kDa
IMMUNOGEN:CARD9 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CARD9.

The immunogen is located within the last 50 amino acids of CARD9.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CARD9 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CARD9 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:CARD9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CARD9 Antibody: CANDF2, hCARD9, Caspase recruitment domain-containing protein 9
ACCESSION NO.:AF311287
PROTEIN GI NO.:11066983
OFFICIAL SYMBOL:CARD9
GENE ID:64170

Background

BACKGROUND:CARD9 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-κB. CARD9 is an upstream activator of BCL-10 and NF-κB signaling.
REFERENCES: 1) Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES. CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-κ B. J Biol Chem. 2000;275(52):41082-6. (RD0202)

For Research Use Only