Datasheet

CARD8 Antibody
CATALOG NUMBER: 3187

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:IF
APPLICATIONS:CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 – Human Lung Tissue Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 47 kDa

Observed: 47 kDa
IMMUNOGEN:CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.

The immunogen is located within the last 50 amino acids of CARD8.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CARD8 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CARD8 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CARD8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CARD8 Antibody: NDPP, DACAR, DAKAR, NDPP1, TUCAN, CARDINAL, KIAA0955, Caspase recruitment domain-containing protein 8, Apoptotic protein NDPP1
ACCESSION NO.:AAG50014
PROTEIN GI NO.:12247742
OFFICIAL SYMBOL:CARD8
GENE ID:22900

Background

BACKGROUND:CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.
REFERENCES: 1) Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506.
2) Stil R, Leonardi A, Formisano L, et al. TUCAN/CARDINAL and DRAL participate in a B activation. FEBS 2002;kcommon pathway for modulation of NF- 521:165-9.
3) Razmara M, Srinivasula SM, Wang L, et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J. Biol. Chem. 2002; 277:13952-8.
4) Pathan N, Marusawa H, Krajewska M, et al. TUCAN, an antiapoptotic caspase-assoicated recruitment domain family protein overexpressed in cancer. J. Biol. Chem. 2001; 276:32220-9.

For Research Use Only