Datasheet

CADM3 Antibody
CATALOG NUMBER: 7975

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CADM3 antibody can be used for detection of CADM3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 44, 48 kDa

Observed: 50 kDa
SPECIFICITY:CADM3 antibody is human, mouse and rat reactive. At least three isoforms are known to exist; this antibody will only detect the two longest isoforms. CADM3 antibody is predicted to not cross-react with other members of the CADM protein family.
IMMUNOGEN:CADM3 antibody was raised against a 16 amino acid peptide near the center of human CADM3.

The immunogen is located within amino acids 220 - 270 of CADM3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CADM3 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CADM3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CADM3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Cell adhesion molecule 3, BIgR, NECL1, Necl-1, TSLL1, IGSF4B, synCAM3
ACCESSION NO.:NP_067012
PROTEIN GI NO.:11056046
OFFICIAL SYMBOL:CADM3
GENE ID:57863

Background

BACKGROUND:Cell adhesion molecule 3 (CADM3) is a member of a family of immunoglobulin-like, calcium-independent cell-cell adhesion molecules (1,2). CADM3 localizes to non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes and is thought to play an important role in the formation of synapses, axon bundles and myelinated axons (3). Recently, CADM3 has been shown to suppress the growth and tumorigenic ability of colon cancer cells, indicating that it can act as a tumor suppressor (4).
REFERENCES: 1) Fukuhara H, Kuramochi M, Nobukuni T, et al. Isolation of the TSLL1 and TSLL2 genes, members of the tumor suppressor TSLC1 gene family encoding transmembrane proteins. Oncogene 2001; 20:5401-7.
2) Takai Y, Mioshi J, Ikeda W, et al. Nectins and nectin-like molecules: roles in contact inhibition of cell movement and proliferation. Nat. Rev. Mol. Cell Biol. 2008; 9:603-15.
3) Kakunaga S, Ikeda W, Itoh S, et al. Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes. J. Cell Sci. 2005; 118:1267-77.
4) Raveh S, Gavert N, Spiegel I, et al. The cell adhesion nectin-like (Necl) molecules 1 and 4 suppress the growth and tumorigenic ability of colon cancer cells. J. Biol. Biochem. 2009; 108:326-36.

For Research Use Only