CAD Antibody Cat. No.: 30-324

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Drosophila
IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of Drosophila CAD.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CAD antibody can be used for detection of CAD by ELISA at 1:12500. CAD antibody can be used for detection of CAD by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL:1) Drosophila Cell Lysate
PREDICTED MOLECULAR WEIGHT: 46 kDa

psi-iconProperties
PURIFICATION:Antibody is purified by peptide affinity chromatography method.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:For short periods of storage (days) store at 4˚C. For longer periods of storage, store CAD antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:cad
ALTERNATE NAMES:cad, Dmel_CG1759, 38E.19, CG1759, Cad, S67, cd, anon-WO2004063362.83, CAD, Dmel\CG1759
ACCESSION NO.:NP_599128
PROTEIN GI NO.:19549891
GENE ID:35341
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Cad regulates embryonic abdominal segment formation by zygotically activating expression of knirps (kni) and giant (gt). It plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets, including folded gastrulation (fog), fork head (fkh) and wingless (wg).

ANTIBODIES FOR RESEARCH USE ONLY.

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