Datasheet

CACNA1H Antibody
CATALOG NUMBER: 8029

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CACNA1H antibody can be used for detection of CACNA1H by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 259 kDa

Observed: 260 kDa
SPECIFICITY:CACNA1H antibody is human, mouse and rat reactive. Multiple isoforms of CACNA1H are known to exist.
IMMUNOGEN:CACNA1H antibody was raised against a 16 amino acid peptide near the center of human CACNA1H.

The immunogen is located within amino acids 1010 - 1060 of CACNA1H.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CACNA1H antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CACNA1H antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CACNA1H antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Calcium channel voltage-dependent T type alpha 1H subunit, CACNA1HB, Cav3.2, ECA6, EIG6
ACCESSION NO.:NP_066921
PROTEIN GI NO.:53832009
OFFICIAL SYMBOL:CACNA1H
GENE ID:8912

Background

BACKGROUND:The calcium channel protein CACNA1H is a T-type member of the alpha-1 subunit family that is part of the voltage-dependent calcium channel complex which mediates the influx of calcium ions into the cell upon membrane polarization (1,2). CACNA1H is a subunit of Cav3.2 T-type calcium channel that is involved in neurological disorders such as epilepsy and pain (2,3). CACNA1H associates with the caveolin protein Cav-3 and it is thought that Cav-3 regulates the Protein Kinase A (PKA) modulation of CACNA1H-containing Cav3.2 T-type calcium channels (4).
REFERENCES: 1) Cribbs LL, Lee JH, Yang J, et al. Cloning and characterization of alpha1H from human heart, a member of the T-type CA2+ channel gene family. Circ. Res. 1998; 83:103-9.
2) Sekiguchi F and Kawabata A. T-type calcium channels: functional regulation and implication in pain signaling. J. Pharmacol. Sci. 2013; 122:244-50.
3) McGivern JG. Targeting N-type and T-type calcium channels for the treatment of pain. Drug Discov. Today 2006; 11:245-53.
4) Markandeya YS, Fahey JM, Pluteanu F, et al. Caveolin-3 regulates protein kinase A modulation of the Cav3.2 (a1H) T-type Ca2+ channels. J. Biol. Chem. 2011; 286:2433-2444.

For Research Use Only