Datasheet

CaBP8 Antibody
CATALOG NUMBER: 5861

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CaBP8 antibody can be used for detection of CaBP8 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
IMMUNOGEN:CaBP8 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CaBP8.

The immunogen is located within the first 50 amino acids of CaBP8.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CaBP8 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CaBP8 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CaBP8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CaBP8 Antibody: CABP8, CABP8, Calcium-binding protein 8, Calneuron I, CaBP8
ACCESSION NO.:NP_113656
PROTEIN GI NO.:157743275
OFFICIAL SYMBOL:CALN1
GENE ID:83698

Background

BACKGROUND:CaBP8 Antibody: Calcium binding proteins (CaBP) play a crucial role in the calcium-mediated cellular signal transduction pathway in the central nervous system. The CaBP family shares much similarity with CaM I (calmodulin), and it has been shown that CaBP proteins can substitute functionally for, and possibly augment the function of, CaM I. CaBP8, contains two EF-hand domains for calcium binding and shares 70% homology with CaBP7 and 50% homology with CaM I. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. Both CaBP8 and CaBP7 possess a targeting mechanism that is unique amongst the CaBPs that may contribute to differential functional Ca2+-sensing by these family members.
REFERENCES: 1) Sokal I, Li N, Verlinde CL, et al. Ca2+-binding proteins in the retina: from discovery to etiology of human disease. Biochim. Biophys. Acta.2000; 1498: 233-51.
2) Haeseleer F, Imanishi Y, Sokal I, et al. Calcium-binding proteins: intracellular sensors from the calmodulin superfamily. Biochem. Biophys. Res. Commun.2002; 290:615-23.
3) Ikura M, Osawa M, and Ames JB. The role of calcium-binding proteins in the control of transcription: structure to function. Bioessays2002; 24:625-36.
4) McCue HV, Burgoyne RD, Haynes LP. Membrane targeting of the EF-hand containing calcium-sensing proteins CaBP7 and CaBP8. Biochem. Biophys. Res. Commun.2009; 380:825-31.

For Research Use Only