Specifications
| HOST SPECIES: | Goat |
| SPECIES REACTIVITY: |
Human |
| IMMUNOGEN: |
C9 Antibody was raised against synthetic peptide ETKGEKNFRTEH found at an internal region of C9. |
| TESTED APPLICATIONS: |
ELISA, WB |
| APPLICATIONS: |
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 70kDa band observed in Human Placenta, Kidney and Liver lysates (calculated MW of 63.2kDa according to NP_001728.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3μg/ml. |
| PREDICTED MOLECULAR WEIGHT: |
Approx 70kDa |
Properties
| PURIFICATION: | C9 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | C9 is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. |
| CONCENTRATION: | 500 ug/mL |
| STORAGE CONDITIONS: | Aliquot and store at -20˚C. Minimize freezing and thawing. |
Additional Info
| OFFICIAL SYMBOL: | C9 |
| ALTERNATE NAMES: | C9, complement component 9, complement component C9 |
| ACCESSION NO.: | NP_001728.1 |
| PROTEIN GI NO.: | 1352108 |
| GENE ID: | 735 |
Background and References
| REFERENCES: | 1) Wickham SE, Hotze EM, Farrand AJ, Polekhina G, Nero TL, Tomlinson S, Parker MW, Tweten RK.Mapping the intermedilysin-human CD59 receptor interface reveals a deep correspondence with the binding site on CD59 for complement binding proteins C8alpha and C9. |
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