C-raf Antibody Cat. No.: 5779

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psi-iconSpecifications
HOST SPECIES:Chicken
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)
IMMUNOGEN: C-raf antibody was raised against an 19 amino acid synthetic peptide near the amino terminus of human C-raf.

The immunogen is located within amino acids 30 - 80 of C-raf.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: C-raf antibody can be used for detection of C-raf by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 72 kDa

Observed: 72 kDa

psi-iconProperties
PURIFICATION:C-raf Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:C-raf Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:C-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:RAF1
ALTERNATE NAMES:C-raf Antibody: NS5, CRAF, Raf-1, c-Raf, RAF, Proto-oncogene c-RAF, cRaf
ACCESSION NO.:NP_002871
PROTEIN GI NO.:4506401
GENE ID:5894
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:C-raf Antibody: C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
REFERENCES:1) Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.
2) Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.
3) Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.

ANTIBODIES FOR RESEARCH USE ONLY.

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