Bub1 Antibody Cat. No.: 4229

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.

The immunogen is located within amino acids 40 - 90 of Bub1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 17-208 - A-20 Cell Slide

psi-iconProperties
PURIFICATION:Bub1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bub1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BUB1
ALTERNATE NAMES:Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A
ACCESSION NO.:O43683
PROTEIN GI NO.:8134347
GENE ID:699
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
REFERENCES:1) Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88.
2) Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle2007; 6:1699-704.
3) Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J.2005; 24:1621-33.
4) Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell2004; 16:387-97.

ANTIBODIES FOR RESEARCH USE ONLY.

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