Bu-1 Antibody [AV20] (Biotin) Cat. No.: 99-312

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Chicken
IMMUNOGEN: Chicken peripheral blood lymphocytes and splenocytes
TESTED APPLICATIONS: Flow
APPLICATIONS: Bu-1 Antibody [AV20] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays.
SPECIFICITY: Bu-1

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Biotin
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS/NaN3
CONCENTRATION:0.5 mg/mL
STORAGE CONDITIONS:Store vial at 2-8˚C

psi-iconAdditional Info
ALTERNATE NAMES:ChB6
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer. The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. The monoclonal antibody AV20 does not cross react with turkey cells when analyzed by flow cytometry.

ANTIBODIES FOR RESEARCH USE ONLY.

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