BTK Antibody Cat. No.: 3395

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (100%)
IMMUNOGEN: BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.

The immunogen is located within amino acids 20 - 70 of BTK.
TESTED APPLICATIONS: ELISA, ICC, IF, IHC-P, WB
APPLICATIONS: BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of BTK are known to exist.
POSITIVE CONTROL:1) Cat. No. 1215 - U937 Cell Lysate
 2) Cat. No. 1224 - Daudi Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 76 kDa

Observed: 79 kDa

psi-iconProperties
PURIFICATION:BTK Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BTK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BTK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BTK
ALTERNATE NAMES:BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase
ACCESSION NO.:Q06187
PROTEIN GI NO.:547759
GENE ID:695
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BTK Antibody: Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
REFERENCES:1) Vetrie D, Vorechovsky I, Sideras P, et al. The gene involved in X-linked aγglobulinaemia is a member of the src family of tyrosine kinases. Nature 1993; 361:226-33.
2) Kawakami Y, Kitaura J, Hata D, et al. Functions of Bruton's tyrosine kinase in mast and B cells. J. Leukoc. Biol. 1999; 65(3):286-90.
3) Jefferies CA, Doyla S, Brunner C, et al. Bruton’s tyrosine kinase is a Toll/Interleukin-1 receptor domain-binding protein that participates in nuclear factor kB activation by Toll-like receptor 4. J. Biol. Chem. 2003; 278:26258-26264.
4) Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.

ANTIBODIES FOR RESEARCH USE ONLY.

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