| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Mouse: (100%) |
| IMMUNOGEN: | BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE. The immunogen is located within amino acids 4760 - 4810 of BRUCE. |
| TESTED APPLICATIONS: | ELISA, IHC-P |
| APPLICATIONS: | BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL. Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist. |
| POSITIVE CONTROL: | 1) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| PREDICTED MOLECULAR WEIGHT: | 534 kDa |
Specifications| PURIFICATION: | BRUCE Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | BRUCE Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | BRUCE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | BIRC6 |
| ALTERNATE NAMES: | BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE |
| ACCESSION NO.: | NP_057336 |
| PROTEIN GI NO.: | 153792694 |
| GENE ID: | 57448 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination. |
| REFERENCES: | 1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90. | 2) Chen Z, Naito M, Hori S, et al. A human IAP-gamily gene, apollon, expressed in human brain cancer cells. Biochem. Biophys. Res. Commun. 1999; 264:847-54 | 3) Hao Y, Sekine K, Kawabata A, et al. Apollon ubiquitinates SMAC and Caspase-9, and has an essential cytoprotection function. Nat. Cell Biol. 2004; 6:849-60. |
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