BRUCE Antibody Cat. No.: 3309

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
IMMUNOGEN: BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE.

The immunogen is located within amino acids 4760 - 4810 of BRUCE.
TESTED APPLICATIONS: ELISA, IHC-P
APPLICATIONS: BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist.
POSITIVE CONTROL:1) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT: 534 kDa

psi-iconProperties
PURIFICATION:BRUCE Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BRUCE Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BRUCE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BIRC6
ALTERNATE NAMES:BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE
ACCESSION NO.:NP_057336
PROTEIN GI NO.:153792694
GENE ID:57448
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.
REFERENCES:1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
2) Chen Z, Naito M, Hori S, et al. A human IAP-gamily gene, apollon, expressed in human brain cancer cells. Biochem. Biophys. Res. Commun. 1999; 264:847-54
3) Hao Y, Sekine K, Kawabata A, et al. Apollon ubiquitinates SMAC and Caspase-9, and has an essential cytoprotection function. Nat. Cell Biol. 2004; 6:849-60.

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