BRSK2 Antibody Cat. No.: 4085

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (87%)
IMMUNOGEN: BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2.

The immunogen is located within amino acids 630 - 680 of BRSK2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: BRSK2 antibody can be used for detection of BRSK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:BRSK2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BRSK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BRSK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BRSK2
ALTERNATE NAMES:BRSK2 Antibody: SAD1, SADA, STK29, PEN11B, C11orf7, HUSSY-12, Brain-selective kinase 2
ACCESSION NO.:Q8IWQ3
PROTEIN GI NO.:116241272
GENE ID:9024
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BRSK2 Antibody: BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.
REFERENCES:1) Manning G, Whyte DB, Martinez R, et al. The protein kinase complement of the human genome. Science2002; 298:1912-34.
2) Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J.2004; 23:833-43.
3) Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science2005; 307:929-32.
4) Sabater L, Gomez-Choco M, Saiz A, et al. BR serine/threonine kinase 2: A new autoantigen in paraneoplastic limbic encephalitis. J. Neuroimmunol.2005; 170:186-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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