Datasheet

Bonzo Antibody
CATALOG NUMBER: 2209

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Bonzo antibody can be used for detection of Bonzo by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 38 kDa

Observed: 43 kDa
IMMUNOGEN:Bonzo antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bonzo/STRL33.

The immunogen is located within the first 50 amino acids of Bonzo.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bonzo Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Bonzo Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bonzo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bonzo Antibody: BONZO, CD186, STRL33, TYMSTR, BONZO, C-X-C chemokine receptor type 6, CDw186, CXC-R6
ACCESSION NO.:AAB64221
PROTEIN GI NO.:2253422
OFFICIAL SYMBOL:CXCR6
GENE ID:10663

Background

BACKGROUND:Bonzo Antibody: Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, and CCR2b in the chemokine receptor family have been identified as HIV coreceptors. An orphan G protein-coupled receptor was recently cloned and designated Bonzo, STRL33 and TYMSTR, and identified as HIV and SIV coreceptor. Bonzo/STRL33 serves as coreceptor for SIV, HIV-2 and HIV-1. The messenger RNA of Bonzo/STRL33 is expressed in lymphoid tissues and activated peripheral blood lymphocytes.
REFERENCES: 1) Deng HK, Unutmaz D, KewalRamani VN, et al. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 1997; 388:296-300.
2) Liao F, Alkhatib G, Peden KW, et al. STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J. Exp. Med. 1997; 185:2015-23.
3) Alkhatib G, Liao F, Berger EA, et al. A new SIV co-receptor, STRL33. Nature 1997; 388:238.
4) Loetscher M, Amara A, Oberlin E, et al. TYMSTR, a putative chemokine receptor selectively expressed in activated T cells, exhibits HIV-1 coreceptor function. Curr. Biol. 1997; 7:652-60.

For Research Use Only