Datasheet

BOK Antibody
CATALOG NUMBER: 3357

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%), Chicken: (81%)
TESTED APPLICATIONS:ELISA, IHC-P
APPLICATIONS:BOK antibody can be used for detection of BOK by immunohistochemistry at 5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 10-301 - Human Brain Tissue Slide
SPECIFICITY:At least three isoforms of BOK are known to exist; this antibody will not detect the smallest isoform. BOK antibody is predicted to not cross-react with other Bcl-2 protein family members
IMMUNOGEN:BOK antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BOK.

The immunogen is located within the first 50 amino acids of BOK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BOK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BOK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BOK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BOK Antibody: BOKL, BCL2L9, Bcl-2-related ovarian killer protein, Bcl-2-like protein 9, hBOK
ACCESSION NO.:AAH06203
PROTEIN GI NO.:14210524
OFFICIAL SYMBOL:BOK
GENE ID:666

Background

BACKGROUND:BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.
REFERENCES: 1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3) Hsu SY, Kaipia A, McGee E, et al. bok is a pro-apoptotic Bcl-2 protein with restricted expression in reproductive tissues and heterodimerizes with selective anti-apoptotic Bcl-2 family members. Proc. Natl. Acad. Sci. USA 1997; 94:12401-6
4) Soleymanlou N, Wu Y, Wang JX, et al. A novel Mtd splice isoform is responsible for trophoblast cell death in pre-eclampsia. Cell Death Differ. 2005; 12:441-52.

For Research Use Only