Bnip3L Antibody Cat. No.: 2289

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (100%), Bovine: (100%)
IMMUNOGEN: Bnip3L antibody was raised against a 16 amino acid peptide near the center of human Bnip3L.

The immunogen is located within amino acids 60 - 110 of Bnip3L.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Bnip3L antibody can be used for detection of Bnip3L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of Bnip3L are known to exist.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1305 - Human Kidney Tissue Lysate
 3) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 24 kDa

Observed: 36, 40 kDa

psi-iconProperties
PURIFICATION:Bnip3L Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Bnip3L Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bnip3L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BNIP3L
ALTERNATE NAMES:Bnip3L Antibody: NIX, BNIP3a, BNIP3A, BNIP3H, NIX, Adenovirus E1B19K-binding protein B5, NIP3L
ACCESSION NO.:NP_004322
PROTEIN GI NO.:4138825
GENE ID:665
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bnip3L Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
REFERENCES:1) Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998; 21:230-5
2) Yasuda M, Han JW, Dionne CA, et al. BNIP3α: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res. 1999; 59:533-7
3) Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J. Biol. Chem. 1999; 274:7-10.
4) Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene 1999;18:4523-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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