Bmf Antibody Cat. No.: 3031

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (92%)
IMMUNOGEN: Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf.

The immunogen is located within the first 50 amino acids of Bmf.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 1211 - HepG2 Cell Lysate
 3) Cat. No. 1201 - HeLa Cell Lysate
 4) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 20 kDa

Observed: 22 kDa

psi-iconProperties
PURIFICATION:Bmf Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Bmf Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bmf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BMF
ALTERNATE NAMES:Bmf Antibody: Bcl-2-modifying factor
ACCESSION NO.:NP_277038
PROTEIN GI NO.:15723378
GENE ID:90427
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
REFERENCES:1) Puthalakath H, Villunger A, O'Reilly LA, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 2001; 293:1829-32.
2) Hunt A and Evan G. Apoptosis. Till death us do part. Science 2001; 293:1784-5.

ANTIBODIES FOR RESEARCH USE ONLY.

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