What is the PUMA Protein?
PUMA, also known as the p53 upregulated modulator of apoptosis, is a protein that belongs to the BCL-2 family of proteins.
It plays a critical role in the regulation of cell death and cancer development. PUMA is a pro-apoptotic protein that is activated in response to DNA damage and other cellular stress signals.
It is known to bind to and inhibit anti-apoptotic Bcl-2 proteins (Bax & Bak), thereby promoting the activation of the intrinsic apoptotic pathway. PUMA has been found to be up-regulated in a variety of cancers due to oncogenic stress, oxidative stress, and DNA damage (to name a few),(2) making it an attractive target for cancer therapy.
Why is PUMA Research Important?
The study of PUMA has gained significant attention in recent years, particularly in the field of cancer research and in mitophagy and autophagy research. The ability to accurately detect and measure PUMA levels in cells and tissues is crucial for understanding its role in cell death and its relationship to p53.
Highly Validated PUMA Antibodies from ProSci
To track and measure levels of expressed PUMA in cells, antibodies are regularly used in applications from western blot, ICC, IF, and ELISA. Popular tools used among researchers with more than 90 citations are ProSci’s PUMA rabbit polyclonal antibodies (Cat No. 3041 & Cat No. 3043).
These antibody preparations have been validated for western blot, ELISA, immunocytochemistry, and immunofluorescence. They have been used in various studies to detect PUMA expression in cell lines and in primary tissues, including cancer cell lines and normal tissues.
PUMA antibody (Cat. No. 3043): Immunofluorescence Validation of PUMA in K562 Cell
ProSci PUMA Antibodies: A Research Tool for Scientists
ProSci’s PUMA antibodies have been cited in many research articles in high-impact journals such as Cell Death & Disease, Journal of Neurotrauma, Frontiers in Cellular Neuroscience, and many more.
This highlights the reliability and quality of ProSci’s PUMA antibodies, which have become a valuable tool for researchers studying the role of PUMA in cell death and its relationship to p53.
Below are some recent publications where the antibodies have been used:
- Sabirzhanov B, Matyas J, Coll-Miro M, Yu LL, Faden AI, Stoica BA, Wu J. Inhibition of microRNA-711 limits angiopoietin-1 and Akt changes, tissue damage, and motor dysfunction after contusive spinal cord injury in mice. Cell Death Dis. 2019 Nov 4;10(11):839. doi: 1038/s41419-019-2079-y. PMID: 31685802; PMCID: PMC6828685.
- Sabirzhanov B, Faden AI, Aubrecht T, Henry R, Glaser E, Stoica BA. MicroRNA-711-Induced Downregulation of Angiopoietin-1 Mediates Neuronal Cell Death. J Neurotrauma. 2018 Oct 15;35(20):2462-2481. doi: 1089/neu.2017.5572. Epub 2018 Jul 10. PMID: 29774773; PMCID: PMC6196751.
- Zhang Y, Yan W, Jung YS, Chen X. PUMA Cooperates with p21 to Regulate Mammary Epithelial Morphogenesis and Epithelial-To-Mesenchymal Transition. PLoS One. 2013 Jun 21;8(6):e66464. doi: 1371/journal.pone.0066464. Erratum in: PLoS One. 2020 Aug 7;15(8):e0237624. PMID: 23805223; PMCID: PMC3689819.
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References
- Gao et al. (2011). Puma is a direct transcriptional target of p53 and a suppressor of angiogenesis. Nature Cell Biology. 13(9), p1113-1119.
- https://www.sciencedirect.com/topics/medicine-and-dentistry/puma-protein