Not too long ago, we published The 5 A’s of Antibodies infographic, which provides a brief explanation of all what goes into the process of generating custom antibodies against specific targets. Our next step is to begin providing summaries of topics within each of the 5 A’s through the Antibody Basics series, designed to explain what is going on at the bench here at ProSci. The Antibody Basics will also serve as a buyer’s guide to help you understand the development of the best possible custom antibody for your project.

Feel free to email us at if you have any suggestions for other topics, or would like to see one topic in particular discussed before others!

Table of Contents


Antibody Basics: Anatomy of an Antibody

Antibody Basics: The Antigen

  • Peptide, Proteins
  • Difficult Targets

Antibody Basics: Adjuvants

  • Adjuvants and Their Mechanisms

Antibody Basics: Animals and Their Advantages

  • Animals for Polyclonal Antibody Development
  • Animals for Monoclonal Antibody Development
  • Animals for Single Domain Antibody Development
  • Rabbit Monoclonal Antibodies

Antibody Basics: Antibody Syntheses

  • Polyclonal Antibody Synthesis
  • Monoclonal Antibody Synthesis
  • Monoclonal Antibodies via Single Cell Cloning
  • Single Domain Antibody Synthesis via Phage Display
  • Monoclonal Antibodies via Yeast Display

Antibody Basics: Assay

  • Western Blot
  • ICC
  • IHC
  • Flow Cytometry
  • ChIP


Antibody Basics: Popular Antibody Research Applications

  • Immunotherapy
  • Vaccine Development
  • Autoimmunity
  • Neurological Diseases

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