Biotin Antibody [Hyb-8] Cat. No.: 33-236

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: All species
IMMUNOGEN: Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
TESTED APPLICATIONS: CM, Flow, IF, IHC, WB
APPLICATIONS: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

In situ Nucleic Acid Hybridization

WB: 2-5 ug/ml

Confocal Microscopy



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.


psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.

ANTIBODIES FOR RESEARCH USE ONLY.

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