BimS/EL/L Antibody [10B12] Cat. No.: 36-186

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psi-iconSpecifications
HOST SPECIES:Rat
SPECIES REACTIVITY: Dog, Human, Monkey, Mouse, Rat
IMMUNOGEN: Synthetic peptide corresponding to aa 20-40 of mouse BimL.
TESTED APPLICATIONS: ELISA, Flow, ICC, IHC, IP, WB
APPLICATIONS: ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SPECIFICITY: Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.

psi-iconProperties
PURIFICATION:>95% (SDS-PAGE)
CLONALITY:Monoclonal
ISOTYPE:IgG2a, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:N/A
ALTERNATE NAMES:BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death
ACCESSION NO.:N/A
PROTEIN GI NO.:N/A
GENE ID:N/A
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.

ANTIBODIES FOR RESEARCH USE ONLY.

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