Datasheet

Bim EL Antibody
CATALOG NUMBER: 7421

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 22 kDa

Observed: 24 kDa
SPECIFICITY:Bim EL antibody is human and mouse reactive. Multiple isoforms of Bim are known to exist; this antibody only detects the Bim EL isoform.
IMMUNOGEN:Bim EL antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bim.

The immunogen is located within amino acids 20 - 70 of Bim EL.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bim EL Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Bim EL Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bim EL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bim EL Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11
ACCESSION NO.:NP_619527
PROTEIN GI NO.:20336315
OFFICIAL SYMBOL:BCL2L11
GENE ID:10018

Background

BACKGROUND:Bim EL Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim, also known as Bcl-2-like protein 11, is a pro-apoptotic member of this family and interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Multiple isoforms of Bim are known to exist, with Bim EL being the longest isoform (1,2).
REFERENCES: 1) O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
2) Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

For Research Use Only