Bim Antibody Cat. No.: 3405

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%)
IMMUNOGEN: Anti-BIM antibody (3405) was raised against a peptide corresponding to amino acids near the center of human BIM.

The immunogen is located within amino acids 80-130 of BIM.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: WB: 5 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
 3) Cat. No. 1201 - HeLa Cell Lysate
 4) Cat. No. 1224 - Daudi Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 22kD

Observed: 22kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BIM expression profile in human and mouse cell lines detected by two independent anti-BIM antibodies that recognize different epitopes, 2065 against N-terminus domain and 3405 against central domain.  BIM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

KD validation (Figure 9, 11, 12): Anti-BIM antibody (3405) specificity was further verified by BIM specific knockdown. BIM signal in 293, U937, Jurkat and U266 cells transfected with BIM siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs or shRNA.

Regulated expression validation (Figure 10): BIM expression detected by anit-BIM antibodies (3405) was up-regulated by flavopiridol treatment, which was blocked by Cycloheximide in U266 cells.

ISOFORMS: Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). 3405 only detect isoform EL.

psi-iconProperties
PURIFICATION:Bim Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Bim Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bim antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BCL2L11
ALTERNATE NAMES:Bim Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11
ACCESSION NO.:O43521
PROTEIN GI NO.:18202042
GENE ID:10018
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
REFERENCES:1) O’Connor et al. EMBO J. 1998; 17:384-395.
2) Hsu et al. Mol. Endocrinol. 1998; 12:1432-40.
psi-iconCITATIONS
CITATIONS: 1)Chen et al. CDK inhibitors upregulate BH3-only proteins to sensitize human myeloma cells to BH3 mimetic therapies. Cancer Res. 2012;72(16):4225-37. PMID: 22693249
2)Tsuchiya et al. Bcl-2 homology domain 3-only proteins Puma and Bim mediate the vulnerability of CA1 hippocampal neurons to proteasome inhibition in vivo. Eur J Neurosci. 2011;33(3):401-8.PMID: 21198986
3)Han et al. Regulation of mitochondrial apoptotic events by p53-mediated disruption of complexes between antiapoptotic Bcl-2 members and Bim. J Biol Chem. 2010;285(29):22473-83.PMID: 20404322
4)Chen et al. Bim upregulation by histone deacetylase inhibitors mediates interactions with the Bcl-2 antagonist ABT-737: evidence for distinct roles for Bcl-2, Bcl-xL, and Mcl-1. Mol Cell Biol. 2009;29(23):6149-69. PMID: 19805519
5)Ulett et al. Mechanisms of group B streptococcal-induced apoptosis of murine macrophages. J Immunol. 2005;175(4):2555-62.PMID: 16081829
6)Dai et al. Disruption of Src function potentiates Chk1-inhibitor-induced apoptosis in human multiple myeloma cells in vitro and in vivo. Blood. 2011;117(6):1947-57. PMID: 21148814
7)Yang et al. Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression. J Immunol. 2009 ;182(5):2959-68. PMID: 19234191
8)Han et al. Degradation of Mcl-1 by granzyme B: implications for Bim-mediated mitochondrial apoptotic events. J Biol Chem. 2004;279(21):22020-9. PMID: 15014070
9)Han et al. Disruption of Mcl-1.Bim complex in granzyme B-mediated mitochondrial apoptosis. J Biol Chem. 2005;280(16):16383-92.PMID: 15713684
10)Han et al. Interrelated roles for Mcl-1 and BIM in regulation of TRAIL mediated mitochondrial apoptosis. J Biol Chem.2006;281(15):10153-63.PMID: 16478725
11)Han et al. Functional linkage between NOXA and Bim in mitochondrial apoptotic events. J Biol Chem. 2007;282(22):16223-31.PMID: 17374615
12)Han et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct Hax-1 cleavage. J Biol Chem.2010;285(29:22461-72. PMID: 20388708
13)Fung et al. Induction of autophagy during extracellular matrix detachment promotes cell survival. Mol Biol Cell. 2008;19(3):797-806. PMID: 18094039
14)Chen et al. Targeting SQSTM1/p62 induces cargo loading failure and converts autophagy to apoptosis via NBK/Bik. Mol Cell Biol. 2014;34(18):3435-49.PMID: 25002530
15)Tanno et al. Bim-dependent apoptosis follows IGFBP-5 down-regulation in neuroblastoma cells. Biochem Biophys Res Commun. 2006;351(2):547-52. PMID: 17067554
16)Dai et al. Targeting autophagy in multiple myeloma. Leuk Res. 2017;59:97-104. PMID: 28599191
17)Dai Y. Targeting Autophagy in multiple myeloma. Virginia Commonwealth University VCU Scholars Compass. Dissertations. 2015.PMID:

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