Datasheet

BICD2 Antibody
CATALOG NUMBER: 4677

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:BICD2 antibody can be used for detection of BICD2 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 94 kDa

Observed: 100 kDa
SPECIFICITY:This BICD2 antibody will not cross-react with BICD1.
IMMUNOGEN:BICD2 antibody was raised against a 14 amino acid synthetic peptide from near the center of human BICD2.

The immunogen is located within amino acids 300 - 350 of BICD2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BICD2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BICD2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BICD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BICD2 Antibody: SMALED2, bA526D8.1, KIAA0699, Protein bicaudal D homolog 2, Bic-D 2
ACCESSION NO.:CAI41013
PROTEIN GI NO.:57208854
OFFICIAL SYMBOL:BICD2
GENE ID:23299

Background

BACKGROUND:BICD2 Antibody: BICD2 is the second human homolog discovered to the Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. Similar to the highly homologous protein BICD1, BICD2 can bind to dynein-dynactin complex, primarily through the dynamitin subunit of dynactin. The C-terminus of BICD2 targets the protein to the Golgi complex while the N-terminal domain of BICD2 co-immunoprecipitates with cytoplasmic dynein, suggesting BICD2 plays a role in the dynein-dynactin interaction on the surface of membranous organelles. Mice engineered to overexpress the BICD2 amino terminal domain in neurons developed amyotrophic lateral sclerosis (ALS)-like features such as Golgi fragmentation, neurofilament swelling in proximal axons, etc., suggesting that impaired dynein/dynactin function may explain some of the pathological features observed in ALS patients.
REFERENCES: 1) Holland PM, Milne A, Garka K, et al. Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J. Biol. Chem.2002; 277:16229-40.
2) Hoogenraad CC, Akhmanova A, Howell SA, et al. Mammalian golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes. EMBO J.2001; 4041-54.
3) Teuling E, van Dis V, Wulf PS, et al. A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice. Hum. Mol. Genet.2008; 17:2849-62.

For Research Use Only