Datasheet

BICD1 Antibody
CATALOG NUMBER: 4663

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:BICD1 antibody can be used for detection of BICD1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1224 - Daudi Cell Lysate
SPECIFICITY:At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.
IMMUNOGEN:BICD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BICD1.

The immunogen is located within the last 50 amino acids of BICD1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BICD1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BICD1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BICD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BICD1 Antibody: BICD, Protein bicaudal D homolog 1, Bic-D 1
ACCESSION NO.:NP_001705
PROTEIN GI NO.:51039802
OFFICIAL SYMBOL:BICD1
GENE ID:636

Background

BACKGROUND:BICD1 Antibody: BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells.
REFERENCES: 1) Baens M and Marynen P. A human homologue (BICD1) of the drosophila bicaudal-D gene. Genomics1997; 45:601-6.
2) Matanis T, Akhmanova A, Wulf P, et al. Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex. Nat. Cell Biol.2002; 986-92.
3) Wanshers BF, van de Vorstenbosch R, Schlager MA, et al. A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells. Exp. Cell Res.2007; 313:3408-20.

For Research Use Only