BHLHA15 Antibody Cat. No.: 7529

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (71%)
IMMUNOGEN: BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15.

The immunogen is located within amino acids 60 - 110 of BHLHA15.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: BHLHA15 antibody is human, mouse and rat reactive.
POSITIVE CONTROL:1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 21 kDa

Observed: 24 kDa

psi-iconProperties
PURIFICATION:BHLHA15 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BHLHA15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:BHLHA15
ALTERNATE NAMES:BHLHA15 Antibody: MIST1, BHLHB8, MIST1, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, bHLHa15
ACCESSION NO.:NP_803238
PROTEIN GI NO.:29126247
GENE ID:168620
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
REFERENCES:1) Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13.
2) Pin CL, Rukstalis JM, Johnson C, et al. The bHLH transcription factor MIST1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J. Cell Biol. 2001; 155:519-30.
3) Lemercier C, To RQ, Carrasco RA et al. The basic helix-loop-helix transcription factor Mist1 functions as a transcriptional repressor of myoD. EMBO J. 1998; 17:1412-22.
4) Zhao Y, Johansson C, Tran T, et al. Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. Mol. Endocrinol. 2006; 20:2187-98.

ANTIBODIES FOR RESEARCH USE ONLY.

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