Datasheet

BFAR Antibody
CATALOG NUMBER: 5591

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
IMMUNOGEN:BFAR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BFAR.

The immunogen is located within amino acids 300 - 350 of BFAR.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BFAR Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BFAR Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BFAR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BFAR Antibody: BAR, RNF47, BAR, Bifunctional apoptosis regulator, RING finger protein 47
ACCESSION NO.:NP_057645
PROTEIN GI NO.:7706091
OFFICIAL SYMBOL:BFAR
GENE ID:51283

Background

BACKGROUND:BFAR Antibody: The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.
REFERENCES: 1) Zhang H, Xu Q, Krajewski S, et al. BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins. Proc. Natl. Acad. Sci. USA2000; 97:2597-602.
2) Roth W, Kermer P, Krajewska M, et al. Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways. Cell Death Differ. 2003; 10: 1178-87.

For Research Use Only