Betacellulin Antibody Cat. No.: 38-179

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography employing immobilized hBetacellulin matrix.
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human Betacellulin (0.1 ng/mL), a concentration of ≤ 0.1 μg/mL of this antibody is required.

ELISA:
Indirect:
To detect hBetacellulin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Sandwich
To detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Betacellulin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

psi-iconProperties
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:Betacellulin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:BTC
ALTERNATE NAMES:ProbetacellulinBTC
ACCESSION NO.:P35070
PROTEIN GI NO.:461653
GENE ID:685
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).

ANTIBODIES FOR RESEARCH USE ONLY.

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