| SPECIES: | Human |
| SOURCE SPECIES: | E. coli |
| SEQUENCE: | Ser122-Ala241 |
| FUSION TAG: | Tag Free |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant protein can be used for biological assays. For research use only. |
| BIOLOGICAL ACTIVITY: | ED50 is less than 1.0 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. Specific Activity is greater than 1 x 106 IU/mg. |
Specifications| PURITY: | Greater than 95% as determined by reducing SDS-PAGE. Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| PREDICTED MOLECULAR WEIGHT: | 13.4 kD |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Lyophilized from a 0.2 um filtered solution of 20mM PB, 250mM NaCl, pH 7.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| STORAGE CONDITIONS: | Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7˚C for 2-7 days. Aliquots of reconstituted samples are stable at -20˚C for 3 months. |
| ALTERNATE NAMES: | Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB |
| ACCESSION NO.: | P01138 |
| OFFICIAL SYMBOL: | NGF |
| GENE ID: | 4803 |
| BACKGROUND: | Human beta -Nerve Growth Factor ( beta -NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits alpha, beta , and gamma ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. beta -NGF is a neurotrophic factor that signals through its receptor beta -NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. beta -NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that beta -NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human beta -NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. |
FOR RESEARCH USE ONLY.
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