Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Properties
PURITY:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
PHYSICAL STATE:
Lyophilized
STORAGE CONDITIONS:
The lyophilized Beta-NGF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Beta-NGF stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Beta-NGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of Recombinant Human β-NGF is a non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity.
Specifications
