Datasheet

beta-Galactosidase Antibody
CATALOG NUMBER: 5155

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Bacteria
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal.

The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.
HOST SPECIES:Chicken

Properties

PURIFICATION:beta-Galactosidase Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:beta-Galactosidase antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal
ACCESSION NO.:NP_752394
PROTEIN GI NO.:26246355
OFFICIAL SYMBOL:lacZ
GENE ID:1036228

Background

BACKGROUND:beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
REFERENCES: 1) Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56.

For Research Use Only