Specifications
Properties
Additional Info
Background
Specifications
| SPECIES REACTIVITY: | Bacteria |
|---|---|
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| IMMUNOGEN: | beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal. The immunogen is located within amino acids 20 - 70 of beta-Galactosidase. |
| HOST SPECIES: | Chicken |
Properties
| PURIFICATION: | beta-Galactosidase Antibody is affinity chromatography purified via peptide column. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | beta-Galactosidase antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgY |
| CONJUGATE: | Unconjugated |
Additional Info
| ALTERNATE NAMES: | beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal |
|---|---|
| ACCESSION NO.: | NP_752394 |
| PROTEIN GI NO.: | 26246355 |
| OFFICIAL SYMBOL: | lacZ |
| GENE ID: | 1036228 |
Background
| BACKGROUND: | beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. |
|---|---|
| REFERENCES: | 1) Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56. |
For Research Use Only
