Beta Actin Antibody Cat. No.: 49-377

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Monkey, Mouse, Pig, Rat, Sheep
IMMUNOGEN: Beta Actin antibody was raised against amino acids 359 - 368 of Beta Actin (Human).
TESTED APPLICATIONS: ELISA, IF, IHC, WB
APPLICATIONS: Beta Actin antibody can be used in Western Blot, and immunohistochemistry starting at 10 μg/mL.

psi-iconProperties
PURIFICATION:Immunoaffinity Chromatography
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
CONCENTRATION:0.5 mg/ml
STORAGE CONDITIONS:Store Beta Actin antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:ACTB
ALTERNATE NAMES:ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1
ACCESSION NO.:P60709
PROTEIN GI NO.:46397333
GENE ID:60
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

ANTIBODIES FOR RESEARCH USE ONLY.

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