Datasheet

Beta-actin Antibody
CATALOG NUMBER: 3777

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Sheep: (100%), Rabbit: (100%), Pig: (100%), Horse: (100%), Chicken: (100%), Guinea pig: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Beta-actin antibody can be used for the detection of Beta-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 41 kDa

Observed: 41 kDa
IMMUNOGEN:Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin.

The immunogen is located within the last 50 amino acids of Beta-actin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Beta-actin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Beta-actin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Beta-actin Antibody: BRWS1, PS1TP5BP1, Actin, cytoplasmic 1, Beta-actin,b-actin
ACCESSION NO.:AAH02409
PROTEIN GI NO.:12803203
OFFICIAL SYMBOL:ACTB
GENE ID:60

Background

BACKGROUND:Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
REFERENCES: 1) Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
2) Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.

For Research Use Only