Datasheet

beta-Actin Antibody [10B7] (biotin)
CATALOG NUMBER: PM-7547-biotin

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Chicken, Drosophila, Human, Mouse, Rabbit, Rat, Zebrafish
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 42 kDa

Observed: 42 kDa
SPECIFICITY:beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
IMMUNOGEN:beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of beta-actin.
HOST SPECIES:Mouse

Properties

PURIFICATION:beta-Actin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:beta-Actin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:beta-Actin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Biotin

Additional Info

ALTERNATE NAMES:Biotin-Actin, Beta-Actin, Actin
ACCESSION NO.:AAH02409
PROTEIN GI NO.:12803203
OFFICIAL SYMBOL:ACTB
GENE ID:60

Background

BACKGROUND:Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
REFERENCES: 1) Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
2) Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.

For Research Use Only